Publications

 

Selected publications from the lab from recent years

 

2021

Constitutive immune mechanisms: mediators of host defence and immune regulation. Paludan SR, Pradeu T, Masters SL, Mogensen TH. Nat Rev Immunol. 2021; 21(3):137-150. doi: 10.1038/s41577-020-0391-5.

Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic stromal keratitis in mice. Yin D, Ling S, Wang D, Dai Y, Jiang H, Zhou X, Paludan SR, Hong J, Cai Y. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-00781-8.

Brain immune cells undergo cGAS/STING-dependent apoptosis during herpes simplex virus type 1 infection to limit type I IFN production. Reinert LS, Rashidi AS, Tran DN, Katzilieris-Petras G, Hvidt AK, Gohr M, Fruhwürth S, Bodda C, Thomsen MK, Vendelbo MH, Khan AR, Hansen B, Bergström P, Agholme L, Mogensen TH, Christensen MH, Nyengaard JR, Sen GC, Zetterberg H, Verjans GM, Paludan SR. J Clin Invest. 2021; 131(1):e136824. doi: 10.1172/JCI136824.

 

2020

Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Hait AS, Olagnier D, Sancho-Shimizu V, Skipper KA, Helleberg M, Larsen SM, Bodda C, Moldovan LI, Ren F, Brinck Andersen NS, Thomsen MM, Freytag MR, Darmalinggam S, Parkes I, Kadekar DD, Rahbek SH, van der Horst D, Kristensen LS, Eriksson K, Kjems J, Mostowy S, Christiansen M, Mikkelsen JG, Brandt CT, Paludan SR, Mogensen TH. Sci Immunol. 2020; 5(54):eabc2691. doi: 10.1126/sciimmunol.abc2691.

SARS-CoV2-mediated suppression of NRF2-signaling reveals potent antiviral and anti-inflammatory activity of 4-octyl-itaconate and dimethyl fumarate. Olagnier D, Farahani E, Thyrsted J, Blay-Cadanet J, Herengt A, Idorn M, Hait A, Hernaez B, Knudsen A, Iversen MB, Schilling M, Jørgensen SE, Thomsen M, Reinert LS, Lappe M, Hoang HD, Gilchrist VH, Hansen AL, Ottosen R, Nielsen CG, Møller C, van der Horst D, Peri S, Balachandran S, Huang J, Jakobsen M, Svenningsen EB, Poulsen TB, Bartsch L, Thielke AL, Luo Y, Alain T, Rehwinkel J, Alcamí A, Hiscott J, Mogensen TH, Paludan SR, Holm CK. Nat Commun. 2020 Oct 2;11(1):4938. doi: 10.1038/s41467-020-18764-3. Erratum in: Nat Commun. 2020; 11(1):5419.

STEEP mediates STING ER exit and activation of signaling. Zhang BC, Nandakumar R, Reinert LS, Huang J, Laustsen A, Gao ZL, Sun CL, Jensen SB, Troldborg A, Assil S, Berthelsen MF, Scavenius C, Zhang Y, Windross SJ, Olagnier D, Prabakaran T, Bodda C, Narita R, Cai Y, Zhang CG, Stenmark H, Doucet CM, Noda T, Guo Z, Goldbach-Mansky R, Hartmann R, Chen ZJ, Enghild JJ, Bak RO, Thomsen MK, Paludan SR. Nat Immunol. 2020; 21:868-879. doi: 10.1038/s41590-020-0730-5.

HSV1 VP1-2 deubiquitinates STING to block type I interferon expression and promote brain infection. Bodda C, Reinert LS, Fruhwürth S, Richardo T, Sun C, Zhang BC, Kalamvoki M, Pohlmann A, Mogensen TH, Bergström P, Agholme L, O'Hare P, Sodeik B, Gyrd-Hansen M, Zetterberg H, Paludan SR. J Exp Med. 2020; 217(7). pii: e20191422. doi: 10.1084/jem.20191422.

 

2019

DNA-stimulated cell death: implications for host defence, inflammatory diseases and cancer. Paludan SR, Reinert LS, Hornung V. Nat Rev Immunol. 2019 Mar;19(3):141-153. doi: 10.1038/s41577-018-0117-0.

Intracellular bacteria engage a STING-TBK1-MVB12b pathway to enable paracrine cGAS-STING signalling. Nandakumar R, Tschismarov R, Meissner F, Prabakaran T, Krissanaprasit A, Farahani E, Zhang BC, Assil S, Martin A, Bertrams W, Holm CK, Ablasser A, Klause T, Thomsen MK, Schmeck B, Howard KA, Henry T, Gothelf KV, Decker T, Paludan SR. Nat Microbiol. 2019 Feb 25. doi: 10.1038/s41564-019-0367-z.

 

2018

STING agonists enable antiviral cross-talk between human cells and confer protection against genital herpes in mice. Skouboe MK, Knudsen A, Reinert LS, Boularan C, Lioux T, Perouzel E, Thomsen MK, Paludan SR. PLoS Pathog. 2018 Apr 2;14(4):e1006976. doi: 10.1371/journal.ppat.1006976.

Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1. Prabakaran T, Bodda C, Krapp C, Zhang BC, Christensen MH, Sun C, Reinert L, Cai Y, Jensen SB, Skouboe MK, Nyengaard JR, Thompson CB, Lebbink RJ, Sen GC, van Loo G, Nielsen R, Komatsu M, Nejsum LN, Jakobsen MR, Gyrd-Hansen M, Paludan SR. EMBO J. 2018 Apr 13;37(8). pii: e97858. doi: 10.15252/embj.201797858.

 

2017

Cutting Edge: Genetic Association between IFI16 Single Nucleotide Polymorphisms and Resistance to Genital Herpes Correlates with IFI16 Expression Levels and HSV-2-Induced IFN-β Expression. Eriksson K, Svensson A, Hait AS, Schlüter K, Tunbäck P, Nordström I, Padyukov L, Liljeqvist JÅ, Mogensen TH, Paludan SR. J Immunol. 2017 Oct 15;199(8):2613-2617. doi: 10.4049/jimmunol.1700385.

Human B cells fail to secrete type I interferons upon cytoplasmic DNA exposure. Gram AM, Sun C, Landman SL, Oosenbrug T, Koppejan HJ, Kwakkenbos MJ, Hoeben RC, Paludan SR, Ressing ME. Mol Immunol. 2017 Nov;91:225-237. doi: 10.1016/j.molimm.2017.08.025. 

Live and let die: ZBP1 senses viral and cellular RNAs to trigger necroptosis. Assil S, Paludan SR. EMBO J. 2017 Sep 1; 36(17):2470-2472. doi: 10.15252/embj.201797845.

cGAS is activated by DNA in a length-dependent manner. Luecke S, Holleufer A, Christensen MH, Jønsson KL, Boni GA, Sørensen LK, Johannsen M, Jakobsen MR, Hartmann R, Paludan SR. EMBO Rep. 2017 Oct; 18(10):1707-1715. doi: 10.15252/embr.201744017.

Inborn errors in RNA polymerase III underlie severe varicella zoster virus infections. Ogunjimi B, Zhang SY, Sørensen KB, Skipper KA, Carter-Timofte M, Kerner G, Luecke S, Prabakaran T, Cai Y, Meester J, Bartholomeus E, Bolar NA, Vandeweyer G, Claes C, Sillis Y, Lorenzo L, Fiorenza RA, Boucherit S, Dielman C, Heynderickx S, Elias G, Kurotova A, Auwera AV, Verstraete L, Lagae L, Verhelst H, Jansen A, Ramet J, Suls A, Smits E, Ceulemans B, Van Laer L, Plat Wilson G, Kreth J, Picard C, Von Bernuth H, Fluss J, Chabrier S, Abel L, Mortier G, Fribourg S, Mikkelsen JG, Casanova JL, Paludan SR, Mogensen TH. J Clin Invest. 2017 Sep 1; 127(9):3543-3556. doi: 10.1172/JCI92280.

IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP. Jønsson KL, Laustsen A, Krapp C, Skipper KA, Thavachelvam K, Hotter D, Egedal JH, Kjolby M, Mohammadi P, Prabakaran T, Sørensen LK, Sun C, Jensen SB, Holm CK, Lebbink RJ, Johannsen M, Nyegaard M, Mikkelsen JG, Kirchhoff F, Paludan SR, Jakobsen MR. Nat Commun. 2017 Feb 10;8:14391. doi: 10.1038/ncomms14391.

 

2016

Sensing of HSV-1 by the cGAS-STING pathway in microglia orchestrates antiviral defence in the CNS. / Reinert, Line S; Lopušná, Katarína; Winther, Henriette; Sun, Chenglong; Thomsen, Martin K; Nandakumar, Ramya; Mogensen, Trine H; Meyer, Morten; Vægter, Christian; Nyengaard, Jens R; Fitzgerald, Katherine A; Paludan, Søren RNat Commun. Vol. 7, 10.11.2016, p. 13348.

Lack of immunological DNA sensing in hepatocytes facilitates hepatitis B virus infection. / Thomsen, Martin K; Nandakumar, Ramya; Stadler, Daniela; Malo, Antje; Valls, Roser Marin; Wang, Fan; Reinert, Line S; Dagnaes-Hansen, Frederik; Hollensen, Anne Kruse; Mikkelsen, Jacob Giehm; Protzer, Ulrike; Paludan, Søren RHepatology. Vol. 64, No. 3, 16.06.2016, p. 746-759.

An innate antiviral pathway acting before interferons at epithelial surfaces. Iversen MB, Reinert LS, Thomsen MK, Bagdonaite I, Nandakumar R, Cheshenko N, Prabakaran T, Vakhrushev SY, Krzyzowska M, Kratholm SK, Ruiz-Perez F, Petersen SV, Goriely S, Bibby BM, Eriksson K, Ruland J, Thomsen AR, Herold BC, Wandall HH, Frische S, Holm CK, Paludan SR. Nat Immunol. 2016 Feb;17(2):150-8. doi: 10.1038/ni.3319.

Innate Antiviral Defenses Independent of Inducible IFNα/β Production. Paludan SR. Trends Immunol. 2016 Sep;37(9):588-96. doi: 10.1016/j.it.2016.06.003. Review

Influenza A virus targets a cGAS-independent STING pathway that controls enveloped RNA viruses. Holm, Christian K; Rahbek, Stine H; Gad, Hans Henrik; Bak, Rasmus O; Jakobsen, Martin R; Jiang, Zhaozaho; Hansen, Anne Louise; Jensen, Simon K; Sun, Chenglong; Thomsen, Martin K; Laustsen, Anders; Nielsen, Camilla G; Severinsen, Kasper; Xiong, Yingluo; Burdette, Dara L; Hornung, Veit; Lebbink, Robert Jan; Duch, Mogens; Fitzgerald, Katherine A; Bahrami, Shervin; Mikkelsen, Jakob Giehm; Hartmann, Rune; Paludan, Søren R. Nat Commun. Vol. 7, 2016, s. 10680